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Rasor Research Group

Publications

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2025

  1. Rasor BJ#, Giaveri S#, Küffner AM#, Erb TJ. “Building complex biochemicals from one-carbon compounds” (in press at Nature Synthesis).
  2. Rasor BJ* and Erb TJ (2025) “Cell-free systems to mimic and expand metabolism” ACS Synthetic Biology, 14(2): 316-322.

2020-2024

  1. Hunt AC#, Rasor BJ#, Seki K, Ekas HM, Warfel KF, Karim AS, Jewett MC (2024) “Cell-free Gene Expression: Methods and Applications” Chemical Reviews, 125(1): 91-149.
  2. Yi X#, Rasor BJ#, Boadi N, Karim AS, Jewett MC, Alper HS (2023) “Establishing a versatile toolkit of flux-enhanced strains and cell extracts for pathway prototyping” Metabolic Engineering, 80: 241-253.
  3. Jung JK#, Rasor BJ#, Rybnicky GA#, Silverman AS#, Granito T, Kuhn RM, Standeven J, Wang BM, Ekas HM, Karim AS, Lucks JB, Jewett MC (2023) “At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring” ACS Synthetic Biology, 12(10): 2909-2921.
  4. Rasor BJ, Karim AS, Alper HS, Jewett MC (2023) “Cell extracts from bacteria and yeast retain metabolic activity after repeated thawing and extended storage” ACS Synthetic Biology, 12(3): 904-908.
  5. Martin J, Rasor BJ, DeBonis J, Karim AS, Jewett MC, Tyo KEJ, Broadbelt L (2023) “A dynamic kinetic model captures cell-free metabolism for improved butanol production” Metabolic Engineering, 76: 133-145.
  6. Rasor BJ#, Chirania P#, Rybnicky GA#, Engle N, Giannone RJ, Tschaplinski T, Karim AS, Hettich RL, Jewett MC (2023) “Mechanistic insights from an integrated -omics analysis of extract preparation methods for cell-free gene expression” ACS Synthetic Biology, 12(2): 405-418.
  7. Liew FM#, Nogle R#, Abdalla T#, Rasor BJ, Canter C, Jensen RO, Wang L, Strutz J, Chirania P, De Tissera S, Mueller AP, Ruan Z, Gao A, Tran L, Engle NL, Bromley JC, Daniell J, Conrado R, Tschaplinski T, Giannone RJ, Hettich RL, Karim AS, Simpson SD, Brown SD, Leang C, Jewett MC, Köpke M (2022) “Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale” Nature Biotechnology, 40: 335-344. Media Highlights: News & Views; Science; Forbes; GEN; Northwestern
  8. Rasor BJ, Vögeli B, Jewett MC, Karim AS (2022) “Cell-free Protein Synthesis for High-throughput Biosynthetic Pathway Prototyping” Methods in Molecular Biology, vol 2433.
  9. Rasor BJ, Yi X, Brown S, Alper HS, Jewett MC (2021) “An integrated cell/cell-free metabolic engineering approach to produce value-added compounds” Nature Communications 12, 5139. Media Highlight.
  10. Meyer C, Namura Y, Rasor BJ, Karim AS, Jewett MC, Tan C (2021) “Analysis of the innovation trend in cell-free synthetic biology” Life, 11(6):551.
  11. Heijstra BD, Fackler N, Rasor BJ, Brown S, Martin J, Ni Z, Shebek KM, Rosin RR, Simpson SD, Tyo KE, Giannone RJ, Hettich RL, Tschaplinski T, Leang C, Brown SD, Jewett MC, Köpke M (2021) “Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation” Annual Review of Chemical and Biomolecular Engineering, 12.
  12. Rasor BJ, Vögeli B, Landwehr GM, Bogart JW, Karim AS, Jewett MC (2021) “Toward sustainable, cell-free biomanufacturing” Current Opinion in Biotechnology, 69.
  13. Karim AS#, Liew FM#, Garg S, Vögeli B, Rasor BJ, Gonnot A, Pavan M, Juminaga A, Köpke M, Jewett MC (2020) “Modular cell-free expression plasmids to accelerate biological design in cells” Synthetic Biology, 5(1).
  14. Grubbe WS#, Rasor BJ#, Krüger A, Karim AS, Jewett MC (2020) “Cell-free styrene biosynthesis at high titers” Metabolic Engineering, 61. Media Highlight.
  15. Karim AS, Dudley QM, Juminaga A, Yuan Y, Crowe SA, Heggestad JT, Abdalla T, Grubbe WS, Rasor BJ, Coar D, Toculas M, Krein M, Liew FM, Quattlebaum A, Jensen RO, Stuart J, Simpson SD, Köpke M, Jewett MC (2020) “In vitro prototyping and rapid optimization of biosynthetic enzymes for cellular design” Nature Chemical Biology, 16. Media Highlight.
  16. Karim AS#, Rasor BJ#, Jewett MC (2020) “Enhancing control of cell-free metabolism through pH modulation” Synthetic Biology, 5(1).

Before 2020

  1. Phelan RM, Sachs D, Petkiewicz SJ, Barajas JF, Blake-Hedges J, Thompson MG, Reider Apel A, Rasor BJ, Katz L, Keasling JD (2017) “Development of next generation synthetic biology tools for use in Streptomyces venezuelae” ACS Synthetic Biology, 6(1).
  2. Hoang TV, Horowitz ER, Chaffee BR, Bruney DG, Flake RE, Rasor BJ, Wagner BD, Robinson ML (2017) “Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity” Epigenetics, 12(1): 27-40.